![]() Calculate the CFU/100 mL in the original culture. Then, 0.1 mL of this dilution was plated out, yielding 73 colonies. A culture was diluted by adding a 0.1 mL aliquot to 0.9 mL water.What is the CFU/mL in the original specimen? A sample was diluted by placing a 0.1 mL aliquot into 0.9 mL of diluent, and 1 mL of this dilution was pour-plated.After incubation at 37C, 279 colonies appeared. 0.1 mL of urine plated out on nutrient agar.PROBLEMS: (Note that they get more challenging as you work your way through them.) The answers to the problems can be found at the bottom of the page. of colonies x dil’n factor x standard unit volume/aliquot plated Remember, all these calculations for calculating microbe per unit volume from the results of a plate count depend on this equation:ĬFU/standard unit volume = no. ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |